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. 2013 Jul 26;8(7):e70712. doi: 10.1371/journal.pone.0070712

Figure 4. Effect of SAHA treatment on saturated and monounsaturated VLCFA levels in Abcd1-deficient U87 astrocytes and B12 oligodendrocytes.

Figure 4

Cells were incubated with serum-containing media with 5 µM SAHA. Fatty acid methyl ester was prepared directly from cells as described in Material and Methods. FAs were analyzed by GC after adding C27∶0 as an internal standard. C26.0, C26∶1, and C22∶0 were measured as a percent of total FAs and expressed as ratio of C26∶0/22∶0 (A and C) and C26∶1/22∶0 (B and D) in Abcd1-deficient U87 astrocytes (A and B) and B12 oligodendrocytes (C and D). Results represent the means ±SD of duplicates from three different experiments. C26∶0 as percentage of total fatty acids (E) and ratio of C26∶0/22∶0 (F) in wild type (WT), Abcd1-KO and SAHA-treated Abcd1-KO mice. Results represent the means ±SE of duplicates from three different animals. *P<0.001; **P<0.05, ***P<0.005, NT-treated; SA5, SAHA (5 µM).