Figure 8. Modulation of apoptotic response in Abcd1-deficient U87 astrocytes and B12 oligodendrocytes.

Cells were treated with cytokines/SAHA in serum-free media and harvested after 24 h. Cell lysate was prepared from control, NT, and untreated/treated Abcd1-deficient U87 astrocytes and B12 oligodendrocytes as described in methods section. (A) Representative immunoblots depict the levels of Bcl₂, Bad, Bax and β-actin protein in Abcd1-deficient U87 astrocytes. (B) Representative immunoblots depict the levels of Bcl₂, BcL-xL, Bax, Bad, Bim, Bik and β-actin protein in Abcd1-deficient B12 oligodendrocytes. SA5, SAHA (5 µM).