Figure 9. Caspase-9 and caspase-3 are involved in the Abcd1-deficiency-induced apoptotic response.

Cells were treated with cytokines/SAHA in serum-free media and harvested after 24 h. Cell lysate was prepared from control, NT, and untreated/treated Abcd1-deficient U87 astrocytes and B12 oligodendrocytes as described in methods section. Representative immunoblots depict the levels of cleaved caspase-9, cleaved caspase-3 and β-actin protein in Abcd1-deficient U87 astrocytes (A) and Abcd1-deficient B12 oligodendrocytes (B). Propidium iodide staining in Abcd1-deficient B12 oligodendrocytes (C), treated with cytokines (D), and treated with SAHA (5 µM) + Cytokines (E) for 24 h in SF media. SA5, SAHA (5 µM).