Table 1. Primers for genome cloning and mutagenesis of IBDV.a .
| Name | Sequence | Orientation | Position (nt) |
| GxAA854GU | AGTCTCAGCgTCGGGGGAGA | sense | A: 845∼864 |
| GxAA854GL | TCTCCCCCGAcGCTGAGACT | antisense | A: 845–864 |
| GxAA876GU | TCGTGTTTCgAACAAGCGTC | sense | A: 867∼886 |
| GxAA876GL | GACGCTTGTTcGAAACACGA | antisense | A: 867∼886 |
| GxA889896U | TCCATGGCCTTgTACTGGGTGC | sense | A: 885∼906 |
| GxA889896L | GCACCCAGTAcAAGGCCATGGA | antisense | A: 885∼906 |
| GxAG938AU | TGATGGGACTaCGGTAATCACC | sense | A: 928∼949 |
| GxAG938AL | GGTGATTACCGtAGTCCCATCA | antisense | A: 928∼949 |
| GxA965980U | TGTGGCCGCAaACAATGGGCTAACGACCGGCACTGA | sense | A: 955∼990 |
| GxA965980L | TCAGTGCCGGTCGTTAGCCCATTGTtTGCGGCCACA | antisense | A: 955∼990 |
| GtAG876AU | TCGTGTTTCgAACAAGCGTC | sense | A: 867∼886 |
| GtAG876AL | GACGCTTGTTcGAAACACGA | antisense | A: 867∼886 |
| GtAG896AU | TCCAAGGCCTTgTACTGGGTGC | sense | A: 885∼906 |
| GtAG896AL | GCACCCAGTAcAAGGCCTTGGA | antisense | A: 885∼906 |
| GxAU | GGAATTCGGATACGATCGGTCTGAC | sense | A: 1∼18 |
| GxA1477L | AGGTAGCCCATGTCTGGT | antisense | A: 1460∼1477 |
| B3P | ACTACCCACTCCTGAACAAA | sense | B:2009∼2028 |
| B37 | GCTCTAGAGGGGGCCCCCGCAGGCGAAGGCCGGGGAT | antisense | B: 2799∼2827 |
a
The positions where the primers GtAG876AU, GtAG876AL, GtAG896AU, and GtAG896AL bind are in accordance with the published sequence of IBDV strain Gt (GenBank assession nos: DQ403248). The positions where other primers bind are in accordance with the published sequence of strain Gx (GenBank assession nos: AY444873 and AY705393). The introduced mutations are lower-case characters. Orientation and position of the primers are shown.