Figure 8. LRP1 modulates SHP-2-mediated migration in response to PDGF-B.

LRP expressing (B41) and deficient (LRP^−/−) fibroblasts were seeded onto 5 µm costar transwell filters at 2×10⁴ cells per well either in the presence or absence of the SHP-2 inhibitor, NSC-87877. After 3 h incubation at 37°C, either buffer (control) or PDGFB (30 ng/ml) was applied to the bottom chamber and incubation continued for 4 hours at 37°C. After incubation, the topsides of filters were cleared of cells with a cotton swab, and cells remaining on the bottom of the filters were fixed and the nuclei were stained with DAPI overnight. Filters were mounted onto glass slides and nuclei were quantified. (*p = 0.02, **p<0.0001, Students t-test).