Skip to main content
. 2013 Jul 26;8(7):e69911. doi: 10.1371/journal.pone.0069911

Figure 2. DHR induces human plasma cell death by activating apoptotic pathway.

Figure 2

A, Human plasma cells LP1 and KMS11 were treated with DHR at the indicated concentrations for 24 h. Cell lysates were then prepared and subject to immunoblotting assay against apoptosis-associated proteins caspase-3, -8 and -9. GAPDH were used as a loading control. B, LP1 and KMS11 cells were treated for 24 h with DMSO, DHR, z-VAD-fmk or DHR+Z-VAD-fmk, followed by caspase-3 analysis by Western blotting. C, LP1 and KMS11 cells were treated for 24 h with DMSO, DHR, z-VAD-fmk or DHR+Z-VAD-fmk, followed by Annexin-V-FITC/PI staining and flow cytometric analyses. Pro-casp: pro-caspase; Cle-casp: cleaved caspase.