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The effect of hRPA on the 3′→5′ exonuclease activity of WRN. Unmodified DNA substrate was incubated without enzyme or with WRN (360 fmol) in the presence of increasing concentrations of hRPA (50–2000 fmol). As a control the DNA substrate was also incubated with hRPA (2000 fmol) in the absence of WRN. The exonuclease reaction products were analyzed and visualized as described earlier.
