Skip to main content
. 2013 Jul 26;8(7):e70636. doi: 10.1371/journal.pone.0070636

Figure 1. Purification of actin isoforms.

Figure 1

(A) SDS-PAGE of purified actin isoforms. (B) Immunoblot-based identification of actin isoforms. The actin antibodies used show no cross-reactivity. (C) Actin isoforms display subtle differences primarily located at their N-terminus as shown in the protein sequence alignment. Sequence variations at the N-terminus of α-, β- and γ-actin are marked in grey. Mature actin isoforms are acetylated (Ac) at their N-terminus (D) 2D-gelelectrophoresis of purified γ-actin (IEP 5.31) containing ∼10% co-purified insect actin (IEP 5.29). The acidic (+) and the basic end (−) are marked.