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. 2013 Jul 26;8(7):e71035. doi: 10.1371/journal.pone.0071035

Figure 1. Y416 is phosphorylated in closed, repressed c-Src.

Figure 1

AD293 cells were transfected with the indicated constructs and after 24h analysed by Western Blots as shown. Key: Em, Emerald fluorescent protein; closed, Q528E, P529E, G530I mutant of c-Src; open, Y527F mutant of c-Src; β-gal, β -galactosidase in same vector as c-Src constructs A. The blot was standardized to c-Src levels. Phospho-Y416 was detected with two different antibodies: #1, cat. PK1109 from Calbiochem; #2, cat. 2101 from Cell Signaling. B. The blot was standardized to endogenous STAT3. Blots were performed twice and showed consistent results.