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. 2013 Jul 26;8(7):e71035. doi: 10.1371/journal.pone.0071035

Figure 4. Expression-level dependence of c-Src on Y416 phosphorylation.

Figure 4

A. Western Blots of AD293 cells transfected with c-Src variants for 24 h standardized to β-tubulin. C-Src was transfected at different doses by adjusting the proportion of DNA used in transfections from 100% to 1.56% supplemented with a vector expressing non-fluorescent Y66L GFP derivative. Key: closed, Q528E, P529E, G530I mutant of c-Src; open, Y527F mutant of c-Src. Phospho-Y416 was detected with two different antibodies: #1, cat. PK1109 from Calbiochem; #2, cat. 2101 from Cell Signaling. B. Densitometry analysis of the Western Blot data in panel A for STAT3 phospho-Y705 immunoreactivity, c-Src immunoreactivity and c-Src phospho-Y416 immunoreactivity with antibody #1 (cat. PK1109). Blots shown are representative of three independent experiments. Densitometry analysis is for the blots shown.