Figure 5. Open c-Src forms a minor proportion of high mass complex in cell lysate.

Sedimentation velocity data at low speed (3,000 rpm) by analytical ultracentrifugation of cell lysates of AD293 cells transfected with c-Src-Emerald is shown as raw data (gray incrementing lines), with the first scan shown in red and the last scan in blue. Data are representative of three independent experiments. Cells were transfected with two different doses of c-Src: either 100% c-Src in transfection, or 12.5% c-Src with the remainder of the DNA supplemented with a non-fluorescent Y66L derivative of Emerald. Under these conditions low mass forms of c-Src-Emerald such as monomers and dimers will not sediment. Brackets indicate the loss of fluorescence in the supernatant due to pelleting of high mass complex (>∼1000 S).