Figure 6. Open and closed c-Src predominately form low mass oligomers in cell lysate.

Sedimentation velocity data at high speed (40,000 rpm) by analytical ultracentrifugation of cell lysates of AD293 cells transfected with c-Src-Emerald is shown as raw data (gray incrementing lines), with the first scan shown in red and the last scan in blue. Data shown are representative of three independent experiments. Fits to a c(s) model are shown in magenta dashed lines. Cells were transfected with two different doses of c-Src: either 100% c-Src in transfection, or 12.5% c-Src with the remainder of the DNA supplemented with a non-fluorescent Y66L derivative of Emerald.