Table 2. Plasmids used in this study.
Plasmids | Description | Reference |
Plasmids for use in M. xanthus | ||
pBJ113 | Vector, KanR, galK | [28] |
pCL153 | pilBWA in pBJ113 | This study |
pCL179 | pilQ in pWB425 | This study |
pMY7 | Vector, KanR, galK | Unpublished |
pRW139 | pilN in pWB425 | This study |
pRW141 | pilO in pWB425 | This study |
pRW142 | pilM in pWB425 | This study |
pRW143 | pilC in pWB425 | This study |
pRW151 | pilP in pWB425 | This study |
pWB425 | Vector, KanR, att | [31] |
pWB600 | ΔpilM in pMY7 | This study |
pWB601 | ΔpilN in pMY7 | This study |
pWB602 | ΔpilO in pMY7 | This study |
pWB603 | ΔpilP in pMY7 | This study |
Plasmids for use in Y2H experiment | ||
pCL127 | GAD-MxPilN | This study |
pCL128 | GAD-MxPilP | This study |
pCL131 | GAD-MxPilO | This study |
pCL134 | GBD-MxPilO | This study |
pCL135 | GBD-MxPilN | This study |
pCL136 | GBD-MxPilP | This study |
pCL137 | GAD-MxPilM | This study |
pCL138 | GBD-MxPilM | This study |
pCL141 | GAD-MxPilQc | This study |
pCL142 | GBD-MxPilQc | This study |
pCL150 | GBD-MxPilQ′ | This study |
pCL152 | GAD-MxPilQ′ | This study |
pCL180 | GAD-TtPilN | This study |
pCL181 | GBD-TtPilN | This study |
pCL182 | GAD-TtPilO | This study |
pCL183 | GBD-TtPilO | This study |
pCL184 | GAD-TtPilW | This study |
pCL185 | GBD-TtPilW | This study |
pCL186 | GAD-TtPilQ0 | This study |
pCL187 | GBD-TtPilQ0 | This study |
pCL188 | GAD-TtPilQ01 | This study |
pCL189 | GBD-TtPilQ01 | This study |
pCL192 | GAD-TtPilQ012 | This study |
pCL193 | GBD-TtPilQ012 | This study |
pGAD | pGADT7, AmpR, Leu | Clontech |
pGAD-T | AmpR, Leu | Clontech |
pGBD | pGBKT7, KanR, Trp | Clontech |
pGBD-53 | KanR, Trp | Clontech |