Figure 2. Ficus arrests the cell cycle in SiHa at G₁/S phase and modulates the expression of cell cycle regulatory proteins.

SiHa cells were treated with different concentrations of FR_aq (0–80 µg/ml) for 24 h. (A) Enhanced accumulation of the cells in G₁ phase with a concomitant decrease in S-phase population was observed after treatment with Ficus (as indicated by histograms). Western blot shows the expression levels of p53 and pRb (B) as well as p21 and ppRb (C). Tubulin was used as a loading control. (D, E) Densitometric analysis of the western blot showing fold change in protein levels upon FR_aqtreatment. The bands were quantified by densitometry scanning using ImageJ 1.44p (Wayne Rasband, National Institutes of Health, USA, http://imagej.nih.gov/ij). The data represents mean ± SD of three independent experiments.