Table 1. Results of the general linear mixed model for analysis of the Pinus pinaster seedlings height 18 months after sowing.
Effects | Without accounting for seed mass covariation | Accounting for seed mass covariation | ||||
DF/VarComp | F-ratio/χ2 | P value | DF/VarComp | F-ratio/χ2 | P value | |
Fixed factors | ||||||
Maternal environment [E] | 1, 9 | 20.2 | 0.001 | 1, 9 | 0.8 | 0.387 |
Block (E)a | 10, 30 | 0.7 | 0.731 | 10, 30 | 0.7 | 0.747 |
Tray | 11, 1206 | 27.7 | <0.001 | 11, 1205 | 28.3 | <0.001 |
Germination time | 1, 1206 | 19.2 | <0.001 | 1, 1205 | 16.1 | <0.001 |
Seed mass | 1, 1205 | 30.8 | <0.001 | |||
Random factors | ||||||
Maternal genotype [G] | 1.4±1.4 | 4.1 | 0.021 | 0.9±1.5 | 3.9 | 0.024 |
G×E | 0.5±1.4 | 0.1 | 0.376 | 1.8±1.6 | 2.5 | 0.057 |
Ramet [R] | 2.1±1.5 | 2.9 | 0.044 | 0.6±1.1 | 0.3 | 0.292 |
Cone (R)a | 1.2±1.1 | 1.4 | 0.118 | 1.2±1.1 | 1.7 | 0.096 |
Residual | 53.7±2.2 | 52.8±2.2 |
Seedlings were derived from 10 maternal genotypes clonally replicated in two contrasting maternal environments, one favourable and one unfavourable for pine growth and reproduction. Analyses excluding and including individual seed mass as a covariate are shown. Degrees of freedom (DF) and F-ratios of fixed factors, and variance components (VarComp) and associated χ2 of random factors are shown. Significance (P value) is indicated in bold (P<0.05).
Block was nested within maternal environment and cone was nested within ramet.