Figure 4. Detection of native CfNOS activity from haemocyte lysates.

The protein of CfNOS was immunoprecipitated by polyclonal antibody of rCfNOS, and incubated with the elution buffer, Spd (0.01 mmol L^-1), L-NAME (0.01 mmol L^-1), and SMT (0.01 mmol L^-1), respectively. CfNOS activity was detected by measuring the NO production using L-Arginine as the substrate. The elution buffer was used as blank. Results were expressed as unit activity per milligram protein (U mgprot^-1). Each value was shown as mean ± SD (N = 3) and bars with different letters were significantly different (P < 0.05).