. 2013 Jul 26;8(7):e69158. doi: 10.1371/journal.pone.0069158

Table 1. Sequences of the primers used in the experiment.

Primer	Sequence (5’–3’)	Sequence information
P1 (forward)	AGTTTCCAGTCTCAAGGCGTTAC	Real-time CfNOS primer
P2 (reverse)	CGCGGTTCTTCTGTTCATTCT	Real-time CfNOS primer
P3 (forward)	CAAACAGCAGCCTCCTCGTCAT	Real-time actin primer
P4 (reverse)	CTGGGCACCTGAACCTTTCGTT	Real-time actin primer
P5 (forward)	ATCCTTCCTCCATCTCGTCCT	CfEF-1a specific primer
P6 (reverse)	GGCACAGTTCCAATACCTCCA	CfEF-1a specific primer
F631 (forward)	TGTTGGACTGTGGNGGNCTGGAATT	Homologous cloning primer
R735 (reverse)	GGAGGNACDATCCACACCCAGTC	Homologous cloning primer
3F1 (forward)	TTGGTGCCAGGGACTTCT	CfNOS specific primer
3F2 (forward)	AGTTTCCAGTCTCAAGGCGTTAC	CfNOS specific primer
3F3 (forward)	GGCTGTGAAGTTCTCCGCTAAA	CfNOS specific primer
3F4 (forward)	TGGGATACTCTCCCGCCTGCATAATG	CfNOS specific primer
3F5 (forward)	ACACTCAACCGAACCTGGTGGAGATTC	CfNOS specific primer
3F6 (forward)	CCTCTACTTTGGCTGTAGGCAGAATGATG	CfNOS specific primer
3F7 (forward)	GTAACTCAAAACGGAATCGGAACTT	CfNOS specific primer
5R1 (reverse)	GACTTGGTCTTGTCCCTGTCTTT	CfNOS specific primer
5R2 (reverse)	ACGCCTTGAGACTGGAAACTATG	CfNOS specific primer
5R3 (reverse)	CCCATCTTGGTCGCAATAAC	CfNOS specific primer
5R4 (reverse)	AAATGGTGCCGCAGTAAAT	CfNOS specific primer
5R5 (reverse)	CCATCGGTTCGTGCCGGAAAAATAGTA	CfNOS specific primer
5R6 (reverse)	ACGAGGTTCTCCAACCGGTCGAGTG	CfNOS specific primer
5R7 (reverse)	CTGGGAGGATGGACTGTGTAAGTTGAT	CfNOS specific primer
5R8 (reverse)	GTTTTTACTCACATCCCCGTTCTCCTT	CfNOS specific primer
P7	GGCCACGCGTCGACTAGTACT₁₇	Oligo(dT)-adaptor
P8	GGCCACGCGTCGACTAGTACG₁₀	Oligo(dG)-adaptor
M13-47	CGCCAGGGTTTTCCCAGTCACGAC	pMD18-T simple vector primer
RV-M	GAGCGGATAACAATTTCACACAGG	pMD18-T simple vector primer
RbF (forward)	TGAGTCGACATATGGACGAAAAACAAGTATTCAGCGACAC	CfNOS recombination primer
RbR (reverse)	CAGGCGGCCGCAGATGTCTATTTCCTTGTCGTTAT	CfNOS recombination primer
T7pro	TAATACGACTCACTATAGGG	pEASY-E1 vector primer
T7ter	GCTAGTTATTGCTCAGCGG	pEASY-E1 vector primer