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. 2013 Jul 26;8(7):e69754. doi: 10.1371/journal.pone.0069754

Figure 2. Gene expression profile of fat specific marker genes to assess adipogenesis and reverse adipogenesis.

Figure 2

Gene expression analysis was performed using qRT-PCR and the resulted expression data were normalized to GAPDH for stepwise assessment of adipogenesis and reverse adipogenesis. Gene expression of adipogenic-specific marker genes (A) PPARG and (B) FABP4 is given for different stages of adipogenic differentiation i.e. at day 5, day 10 and day 15. Similarly, the gene expression of (C) PPARG and (D) FABP4 is given for different stages of reverse adipogenesis (dedifferentiation). Error bars, Means ± S.E.M (n = 3); *P<0.05; **P<0.01; ***P<0.001, NS, not significant (student t test performed for statistical analysis).