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. 2013 Jul 26;8(7):e69754. doi: 10.1371/journal.pone.0069754

Table 1. Evaluation of different biological parameters for each cluster.

Cluster 1
Gene ontologies Cellular compartmentalization Molecular function KEGG signaling pathways Functional categories Expression site
GO:0008610∼lipid biosynthetic process (24) GO:0005739∼mitochondrion (59) GO:0048037∼cofactor binding (32) hsa03320:PPAR signaling pathway (13) phosphoprotein (129) Placenta (75)
GO:0006631∼fatty acid metabolic process (23) GO:0031090∼organelle membrane (49) GO:0008289∼lipid binding (22) hsa04910:Insulin signaling pathway (13) acetylation (71) Liver (72)
GO:0032868∼response to insulin stimulus (13) GO:0000267∼cell fraction (48) GO:0031406∼carboxylic acid binding (19) hsa00071:Fatty acid metabolism (8) oxidoreductase (43) Skin (48)
GO:0010876∼lipid localization (8) GO:0005829∼cytosol (48) GO:0000287∼magnesium ion binding (15) hsa01040:Biosynthesis of unsaturated fatty acids (6) transferase (32) Adipose tissue (17)
GO:0055088∼lipid homeostasis (7) GO:0005783∼endoplasmic reticulum (44) GO:0019842∼vitamin binding (14) hsa04920:Adipocytokine signaling pathway (6) lipoprotein (16) Fetal liver (11)
GO:0006869∼lipid transport (7) GO:0031975∼envelope (31) GO:0009055∼electron carrier activity (11) hsa00564:Glycerophospholipid metabolism (6) Apoptosis (14) Fetal brain cortex (11)
GO:0030258∼lipid modification (6) GO:0005794∼Golgi apparatus (30) GO:0005504∼fatty acid binding (9) hsa00062:Fatty acid elongation in mitochondria (4) Acyltransferase (7) Adipocyte (8)
GO:0034440∼lipid oxidation (5) GO:0005615∼extracellular space (22) GO:0004091∼carboxylesterase activity (7) hsa00061:Fatty acid biosynthesis (3) diabetes mellitus (6)
GO:0045444∼fat cell differentiation (5) GO:0005792∼microsome (16) GO:0016229∼steroid dehydrogenase activity (5)
GO:0010883∼regulation of lipid storage (4) GO:0009986∼cell surface (12)

The genes of each cluster were uploaded individually to online databases (DAVID and KEGG) and analyzed for their link to different biological parameters like gene ontology, cellular compartmentalization, molecular function, signaling pathway and site of expression. The parameters were selected on the basis of the enrichment score and relevance for adipogenesis. The numbers given in brackets are the numbers of genes associated to the corresponding GO term and signaling pathways.