Table 2. Primers used in PCR assays to detect key osteoblastogenic and osteoclastogenic regulatory genes and markers.
| Gene Name | Forward Primer | Reverse Primer |
|---|---|---|
| OPG | CCGCGTGTGCGAATGCAAGG | TGGGGTTCCAGCTTGCACCAC |
| RANKL | AAGGAGCTGTGCAAAAGGAATT | TGATGTGCTGTGATCCAACGA |
| Osteocalcin | CTGCGATGACACAGCAAATC | GGACTTTGCCTTCTTCCACA |
| Osterix | TTCTGACTGTCTGCCCAGTG | GCCTTGTACCAGGAGCCATA |
| ALP | CCACGTCTTCACATTTGGTG | GCAGTGAAGGGCTTCTTGTC |
| BMP2 | ACATGGTTGTGGAGGGTTGT | CAACTGGGGTGGGGTTTT |
| BMP3 | TAGAGTCTTGCGCTTGCAGA | GAAACAAAATGCATTGGCAGT |
| BMP4 | TCCATGCTGTACCTGGATGA | GGAACGTGTGTGTGTGGTGT |
| BMP6 | GCGCCAACTAAGCTAAATGC | TCCAAGGCAGAATGTGTGTC |
| BMP7 | TGCCATCTCCGTCCTCTACT | GCAATGGAGGATCCAGAAAA |
| ALK2 | CAAAATCCATCCGCAAGACT | GCTGGACAATGACAACAACG |
| ALK3 | AGCCTCCAGACTCACAGCAT | CATGCCATGGGTAAAAACAG |
| ALK6 | CCTGCGGGTTAAGAAAACAC | CTCTGCCCACAAACAGAAGA |
| BMPRII | TCTTTTCTTTGCCCTCCTGA | AGCAGGATGGTCCATGGTAG |
| Runx2 | TCTGGCCTTCCACTCTCAGT | ATGAAATGCTTGGGAACTGC |