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. 2013 Jul 26;8(7):e69612. doi: 10.1371/journal.pone.0069612

Figure 4. Distributions of GABAergic and glutamatergic postsynaptic sites on dendritic arbors of distinct types of RGCs.

Figure 4

Biolistic transfection of individual ganglion cells in Thy1-YFPγ2 retinas with a cell label (tdTomato) and excitatory postsynaptic protein (PSD95CFP). (A) Representative flat mount (upper) and vertical (lower) MIPs of three distinct RGC types in P12 Thy1-YFPγ2 retina; ON-S A-type, OFF-S A-type, and bistratified DS RGCs. Inserts are 4x magnifications of a region of the arbor showing PSD95CFP and YFPγ2 found only within the dendrites. (B) MIPs of the reconstructed dendritic skeletons and identified postsynaptic sites from the cells in (A). Inserts represent the same regions as inserts in (A). (C, D) Example MIPs of the raw images of P21 cells (C), their skeletonized dendrites and identified postsynaptic sites (D).