Table 2.

RT-qPCR results showing concentrations of putative Con-pgp-9 RNA expression (ag/2 ng total RNA) in isolates before and 10 days after selection with injectable ivermectin at a dose rate of 0.2 mg ivermectin/kg body weight

	Females					Males
Isolate		Median	Mean	SD	Sign. p (95 % CI)	Median	Mean	SD	Sign. p (95 % CI)
All	Before	153	172	124		20	27	25
	After	83	196	250	0.747	44	46	32	0.091
A	Before	122	121	38		42	39	7
	After	458	420	343	0.125	44	44	35	0.764
B	Before	155	154	37		13	29	41
	After	83	108	65	0.213	48	41	21	0.571
C	Before	301	242	203		17	17	9
	After	55	60	33	0.119	40	54	43	0.137

Group A was infected with a propagated IVM-susceptible laboratory isolate, and groups B and C were infected with two different field isolates showing phenotypic clinical IVM resistance in field trials