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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Biomaterials. 2013 Jun 12;34(28):6695–6705. doi: 10.1016/j.biomaterials.2013.05.040

Figure 4. Mineralization of outer scaffolds of wound model.

Figure 4

(A–F) Alizarin Red S staining of outer scaffolds after twelve weeks of culture. At Week 6, scaffolds were treated with: glibenclamide (GL, 10 μM), monensin (MO, 10 nM), barium chloride (BA, 100 μM), high K+ (HK, 40 mM), or a sequential treatment of HK and BA (KB, 3 weeks HK, 3 weeks BA), or were left untreated (OS). Scale bar = 500 μm. (G) Quantification of calcium content of the outer scaffolds. Data points in bar graph are mean calcium content normalized to DNA content (μg Ca per μg DNA) ± standard deviation. A one-way ANOVA test was performed, followed by the Tukey-Kramer post-hoc test. Groups that are not significantly different (p > 0.05) are labeled with the same letter. Red line: Mean normalized calcium content of pre-cultured scaffold at Week 6 (μg Ca per μg DNA) prior to wounding.