Figure 3.

Ph–PNA conjugate uptake measured using an ion-selective electrode. An ion-selective electrode was used to measure the concentration of a ph–PNA conjugate in a mitochondrial suspension. The arrows indicate five sequential additions of 1 µM ph–PNA to calibrate the electrode response. The mitochondria were then energised by addition of succinate (5 mM) and uncoupled with FCCP (10 µM) as indicated.