Table 3.

Routine RHD PCR of blood donors

Blood Service	Testing strategy
Red Cross Blood Service Baden Württemberg - Hesse [28] (Germany)	all first time donors, pooled testing for RHD intron 4
Red Cross Transfusion Service of Upper Austria [30]	all first time donors, pooled testing for RHD intron 4.7.10; no IAT
NIH (USA)	single donor testing (WA Flegel, Washington, personal communication)
Aarhus Hospital (Denmark) [47]	exon 10 for donors with C or E
Red Cross Blood Service NSTOB (Germany)	all first time donors, pooled testing for RHD exon 7
Albert Einstein Hospital, São Paulo (Brazil) [48]
Red Cross Blood Service (Bern, Switzerland)*	pooled testing for exons 3.5.10 (H Hustinx, Bern, personal communication)
Red Cross Blood Service (Zurich, Switzerland)*	pooled testing intron 4 / exons 5 +7 (until 2011), single donor exon 5, 7 and 3’ untranslated (since 2012) (C Gassner, Zurich, personal communication)
Red Cross Blood Service (Innsbruck, Austria)	single donor testing of donors with C or E

^*RHD PCR of D-negative donors is mandatory in Switzerland since January 2013, serologie testing by IAT has been dropped.