A43 has two N-linked oligosaccharides. (A) Expression of wild type and mutated A43HA. BS-C-1 cells were infected with vΔA43GFP and transfected with one of four plasmids expressing wild type A43HA or A43HA with mutations N65Q, N93Q, or N114Q. Cell extracts were analyzed by SDS-PAGE and Western blotting with anti-HA antibody. (B) Partial (+) or mock (−) PNGase digestion of cell extracts at 24 h infection with vΔA43GFP and transfection with pA43HA, pN65Q, pN93Q, or pN114Q. The digests were analyzed by Western blotting with antibody to the HA epitope tag.