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. Author manuscript; available in PMC: 2013 Jul 27.
Published in final edited form as: Virology. 2009 Nov 8;396(1):160–168. doi: 10.1016/j.virol.2009.10.025

FIG. 3.

FIG. 3

A43 has two N-linked oligosaccharides. (A) Expression of wild type and mutated A43HA. BS-C-1 cells were infected with vΔA43GFP and transfected with one of four plasmids expressing wild type A43HA or A43HA with mutations N65Q, N93Q, or N114Q. Cell extracts were analyzed by SDS-PAGE and Western blotting with anti-HA antibody. (B) Partial (+) or mock (−) PNGase digestion of cell extracts at 24 h infection with vΔA43GFP and transfection with pA43HA, pN65Q, pN93Q, or pN114Q. The digests were analyzed by Western blotting with antibody to the HA epitope tag.