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. Author manuscript; available in PMC: 2013 Jul 27.
Published in final edited form as: Virology. 2009 Nov 8;396(1):160–168. doi: 10.1016/j.virol.2009.10.025

FIG. 6.

FIG. 6

Intracellular trafficking of A43. (A) BS-C-1 cells were infected with vΔA43GFP followed by transfection with plasmids expressing the full length HA-tagged A43 (pA43HA) or a truncated version missing the transmembrane domain (pA43HAΔTM). After 24 h, the proteins in the cell lysates (L) or concentrated medium (M) were analyzed by Western blotting with an antibody to the HA tag. (B) HeLa cells were infected with vA43V5 and brefeldin A was added to one culture after 4 h. At 18 h post infection, the cells were allowed to react with a membrane-impermeable biotin reagent. The biotinylated proteins were affinity purified on NeutrAvidin gel beads. Western blotting with V5 antibody was performed on the input (I), unbound (U) and bound and eluted (E) proteins.