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. Author manuscript; available in PMC: 2013 Jul 27.
Published in final edited form as: Virology. 2009 Nov 8;396(1):160–168. doi: 10.1016/j.virol.2009.10.025

FIG. 7.

FIG. 7

Replication of A43R deletion mutant. (A) One-step growth curve. BS-C-1 cells were infected at a multiplicity of 10 PFU/cell with vΔA43GFP and VACV WR. Virus yields were determined at the indicated times post infection by plaque assay. (B) Formation of vΔA43GFP and VACV WR plaques. BS-C-1 cells were infected with vΔA43GFP and VACV WR using a methylcellulose overlay. Cells were fixed and stained with crystal violet at 48 h after infection. (C) Comet formation. BS-C-1 cells were infected with VACV IHD-J or the A43 deletion mutant in the IHD-J background ΔA43GFP using a liquid overlay. After 48 h, the cells were stained as in panel A.