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. 2013 Jul 8;110(30):12391–12396. doi: 10.1073/pnas.1302856110

Fig. 1.

Fig. 1.

AhR is required for proper cytolytic activity by conventional NK cells upon cytokine stimulation. (A) Splenic murine NK cells, enriched by magnetic bead negative selection, were cultured for 24 h in the presence of the indicated cytokines. Expression of AhR was assessed by qRT-PCR and normalized to expression of Hprt. (B) Enriched splenic murine NK cells from AhR−/− and AhR+/+ mice were cultured in IL-2 (1,000 U/mL) for 7 d and then assessed for cytolytic activity against RMA-S target cells in a 4-h calcein-release assay. (C) Fresh splenic murine NK cells (NK1.1+CD3−) from AhR−/− and AhR+/+ mice were assessed for CD27 and CD11b expression by FACS (n = 3 experimental replicates). (D) Fresh splenic murine NK cells (NK1.1+CD3−) from AhR−/− and AhR+/+ mice were assessed for KLRG1 expression by FACS (n = 3–5 mice per group). Results in this figure were reproduced at least once.