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. 2013 Jul 8;110(30):12331–12336. doi: 10.1073/pnas.1222684110

Fig. 1.

Fig. 1.

PER2 suppression promoted tumorigenic ability. (A–C) Immunoblot analysis of PER2 (A), PER1 (B), and PER3 (C) in breast cancer cells (MB-231 and SKBR-3) and normal mammary epithelial cells (MCF-10A and M10). Tubulin was used as a loading control. Relative expression (RE) level of PER protein in SKBR-3, MCF-10A, and M10 relative to MB-231 is indicated. (D and E) Soft agar colony formation (SACF) assay using MCF-10A (D) and SKBR-3 (E) cells infected with lentiviral-control shRNA (sh-Ctrl) or sh-PER2. (F) SACF assay using SKBR-3 cells transduced with lentiviral control (Ctrl) or PER2. PER2 expression in these cells was examined with immunoblot. Tubulin was used as a loading control. Data show means ± SD. *P < 0.05 (Student t test). (G) Tumorigenesis assay of NOD/SCID mice injected with SKBR-3 lentiviral Ctrl or PER2 overexpressing cells. Cell dose: 3 × 106 cells per fat pad. Five mice were used for each group. Data show means ± SD. *P < 0.05 (Student t test) (SI Appendix, Fig. S1).