Table 1.

Best fit parameters of the k_obs,fast substrate dependence

Amino acid	kon (107 M−1 s−1)	koff (s−1)	k1 (s−1)	k2	KD (µM)*
l-aspartate	3.0 ± 0.2†	250 ± 40	506 ± 4	n.d.§	8.3 ± 2.0
	(2.5; 4.1)‡	(150; 400)	(490; 530)		(4.7; 11.8)
d-aspartate	2.8 ± 0.5	1,200 ± 320	750 ± 10	n.d.	43 ± 20
	(1.6; 3.9)	(500; 1,900)	(730; 760)		(8.0; 78)
l-CS	3.9 ± 0.6	41 ± 19	194 ± 5	n.d.	1.1 ± 0.7
	(2.6; 5.1)	(0; 82)	(184; 204)		(0; 2.2)

n.d., not determined.

^*Intrinsic dissociation constant calculated from K_D = k_off/k_on.

^†The SE of the fit is indicated.

^‡Parentheses contain 95% confidence intervals for the measured rate constants and 90% confidence intervals for calculated K_D values.

^§k₂ was fixed to 10 s⁻¹ in all fits.