Fig. 1.
RIG-I–like receptors are not essential for the antiviral response to MLV in primary MEFs. (A and B) Ddx58+/+/Ifih1+/+ and Ddx5−/−/Ifih1−/− MEFs (A) or Irf3+/−/Irf7+/− and Irf−/−/Irf7−/− MEFs (B) were infected with MLV (2 × 1010 copies per μL) for 48 or 72 h. The copy numbers of the MLV genome in the culture supernatants were measured by quantitative RT-PCR. (C–F) Ddx58+/+/Ifih1+/+ and Ddx58−/−/Ifih1−/− MEFs (C and E) or Irf3+/−/Irf7+/− and Irf3−/−/Irf7−/− MEFs (D and F) were infected with MLV (2 × 1010 copies per μL) or VSV [multiplicity of infection (MOI) = 1] for 12 h. The levels of Ifnb1 (C and D) and Cxcl10 (E and F) mRNAs were measured by quantitative RT-PCR. The results shown are means ± SD (n = 3).