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. 2013 Jul 8;110(30):E2838–E2847. doi: 10.1073/pnas.1306097110

Fig. 2.

Fig. 2.

Torins potently inhibit Plasmodium blood stage infection. (A) Effect of Torin1 and Torin2 in P. falciparum blood stage in vitro cultures. Synchronized P. falciparum 3D7 ring stage parasites were treated with 200 nM Torin1, 10 nM Torin2, or DMSO (vehicle control); reinvasion was analyzed after SybrGreen labeling of parasite DNA 48 h later. Data represent mean ± SD of three independent experiments. Starting parasitaemia are represented by dotted line. (B) Dose-dependent effect of Torin2 on P. falciparum blood growth. Data represent mean ± SD of three independent experiments. (C and D) Effect of a single 10-mg/kg oral dose of Torin2 administered on day 4 after infection of C57BL/6 mice with 1 × 106 Pb-GFP–parasitized RBC on survival (C) and parasitemia (D). Animals were monitored daily for malaria-associated pathology (experimental cerebral malaria symptoms), and parasitaemia was analyzed by flow cytometry. (C) Cumulative survival curve for three independent experiments; n = 10 mice for control and Torin2-treated groups. (D) Parasitaemia; each point represents the mean ± SD from three independent experiments.