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. 2013 Jul 8;110(30):E2838–E2847. doi: 10.1073/pnas.1306097110

Fig. 5.

Fig. 5.

Torin2 treatment leaves the PVM structurally intact but lacking PVM-resident proteins. (A and B) Effect of Torin2 treatment on the PVM and UIS4 localization in young liver stage trophozoites. Schematic illustrates experimental setup. (A) Representative images of TEM of 8-h EEFs after Torin2 or DMSO (control) treatment. (B) IFA at 2 h (control, single confocal slice) and at 8 h in control [maximum intensity projection (MIP)] and Torin2-treated cells [single confocal slice (HSP70, green; DAPI, blue; UIS4, red)]. Fluorescence intensity graphs of the trajectory indicated by the white lines in the preaddition and Torin2-treated images show the relative spatial intensity peaks of the three fluorophores. (C) Effect of Torin2 treatment on UIS4 and EXP1 during early liver stage schizogony. Schematic illustrates experimental setup. MIPs of the entire EEF are shown for A–C.