Table 1.
Torin2 is refractory to repeated attempts at resistance selection in vitro
| Selection strategy | Recrudescence of parasites | Days to recrudescence (first/second/third round)* | Fold EC50 (nM)** |
| 10× EC50, 192 h | 0/8 | NA | NA |
| 10x EC50, 144 h | 0/4 | NA | NA |
| 20x EC50, 48 h pulsed three times | 0/4 | NA | NA |
| 20x EC50, 48 h | 1/7 | 26/24/30 | 1.04 |
| 20x EC50, 36 h | 1/3 | 26/23/30 | 0.93 |
| 20x EC50, 24 h | 2/4 | 22/24/31 | 1.14 |
| 20x EC50, 12 h | 0/2 | NA | NA |
Asexual Dd2 parasites were subjected to varied step-wise intermittent selection protocol with10x EC50 Torin2.
*
Number of days for each independent selection to return to 1% parasitemia. The time to recrudescence for subsequent rounds of selection is expressed separately for the first/second/third round of selections and expressed as the average of the number of days when multiple independent selections were performed with identical protocols.
**
Parental Dd2 strain EC50 is 0.7 nM (0.48,1.02). Values expressed as fold change over Dd2 EC50.