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Glial differentiation on collagen-laminin substrates–Glia stained with GFAP (red) on Day 5 (A–D) and Day 15 (E–H) coverslips coated with type I Collagen and 5μg/cm2 Laminin (A,E) or 10μg/cm2 Laminin (B,F) or type IV Collagen with 5 (C,G) or 10 (D,H) μg/cm2 of Laminin. Addition of laminin to collagen substrates promoted glial differentiation both at days 5 and 15. No significant difference was observable in GFAP+ glial differentiation between 5 and 10 μg/cm2 of laminin.
