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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Immunol. 2013 Jul 3;191(3):1260–1275. doi: 10.4049/jimmunol.1300770

Table 1. Comparison of B-cell subset absolute numbers in BM and spleen of WT, and 2F5 or 4E10 complete (VH+/+ × VL+/+) KI mice.

Bone Marrowb
Total B-cells ProB/large pre-B Small pre-B Immature B T1/T2 Mature
Mouse n B220+CD19+c B220loCD43+ B220loCD43− IgMint/hiIgD− IgMhiIgDlo/int IgMintIgDhi
WT 6 82.4±16.0a 15.7±6.8 50.0±16.8 9.7±2.0 1.8±0.6 5.1±1.6
2F5VH+/+×VL+/+ 6 41.7±9.4 15.5±4.8 24.7±8.4 2.5±1.2 0.013±0.005 0.079±0.021
4E10VH+/+×VL+/+ 5 68.2±9.5 12.5±4.5 54.5±7.1 1.7±1.0 0.016±0.007 0.094±0.022
Spleen
Total B-cells NF/T MZ Mature
Mouse n B220+CD19+ CD21−CD23− CD23intCD21hi CD23hiCD21int
WT 8 106.4±18.0 18.1±3.4 5.4±1.6 70.2±13.3
2F5VH+/+×VL+/+ 6 1.9±0.3 0.71±0.20 0.073±0.019 0.50±0.07
4E10VH+/+×VL+/+ 5 4.4±0.7 1.6±0.2 0.016±0.008 0.28±0.07
a

Values are absolute cell numbers (×105±SEM)

b

Bone marrow cells were taken from both femurs for FACS analysis.

c

Cells were gated on singlet/live/lymphocytes. B-cell subset annotations are based on those defined in Figs. 2A, B.