Table 2. Summary of isotypic distribution and MPER reactivities in primary screen of hybridoma clones derived from 2F5 and 4E10 complete KI spleens^a.

Fusion ID	Mice	Total seeded wells	Wells with cell growthb	IgM+c	IgG+	IgA+	MPER+d
2F5-V101	2F5VH+/+ × VL+/+	3040	510	225	0	0	224
4E10-V2	4E10VH+/+ × VL+/+	3200	146	50	0	0	43
4E10-V3	4E10VH+/+ × VL+/+	1600	174	100	0	0	100

^aAll fusions were performed using NS0-bcl2 myeloma fusion partner lines and represent a different individual mouse.

^bCulture plates were screened under microscope for cell growth.

^cIg levels /isotypes of all cloned hybridoma lines were determined by ELISA as described in Materials and Methods. Lines were considered positive if >3× above background OD binding was detected in 1:40 diluted supernatants.

^dMPER reactivity of 2F5 or 4E10 hybridoma lines was determined by ELISA to their nominal MPER epitopes (as specified by SP62 and MPR.03 peptides, respectively). Lines were considered positive if >3× above background OD binding was detected in 1:2 diluted supernatants.