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. Author manuscript; available in PMC: 2013 Jul 27.
Published in final edited form as: Gastroenterology. 2008 Feb 1;134(3):727–737. doi: 10.1053/j.gastro.2007.12.033

Figure 5. GDNF transgenic mice have higher β-cell mass and increased proliferation compared to WT mice.

Figure 5

Figure 5

(A) Pancreatic sections from WT and GDNF-tg mice stained for insulin (red) and counterstained with hematoxylin (blue). Images in the lower panel are higher magnifications of the islets in the upper panel. Arrows show representative islets. Scale bar: 100 µm. Histogram shows comparison of β-cell mass (expressed as percentage insulin stained area/pancreatic area) between WT and GDNF-tg mice (means ± SE, ***, P<0.001). (B) Number of islets per unit pancreatic tissue area in WT and GDNF-tg mice. (C) Islet size of WT and GDNF-tg mice. (D) Comparison of β-cell sizes between WT and GDNF-tg mice (n=26, P>0.05). (E) Total insulin content of pancreata from WT and GDNF-tg mice (male and female, n= 6, *P<0.05). (F) Pancreatic sections from WT and GDNF-tg mice immunostained for Ki67 (green), insulin (red) and DAPI nuclear stain (blue) to show the presence of proliferating β-cells within islets. Higher magnification image of boxed area is shown. Scale bar; 20 µm. Histogram shows a plot of percentage of Ki67+ β-cells (mean ± SE, ***, P<0.001).