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. 2013 Jul 22;10:242. doi: 10.1186/1743-422X-10-242

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Copyright ©2013 Keating et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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WNV NS5, but not tick-borne LGTV NS5, interacts with PKGIβ. HEK293T cells were transfected with DNA plasmids encoding WNV NS5 or LGTV NS5 and PKGIβ-Flag. Total protein from the lysate was subjected to immunoprecipitation using α-Flag. Total cell lysates or immunoprecipitated proteins were subjected to Western blot analysis using α-WNV MTase (for the detection of WNV NS5 (left panel)) and α-HA (for the detection of LGTV NS5 (right panel)), α-Flag (for the detection of PKG), and α-actin to show equal protein loading for the lysate.