Table 1.
Summary of TNF-α PCR-RFLP analysis
|
Variants |
Primer sequence (5′ to 3′) |
PCR products |
Anneal T |
|
Allele |
|
|---|---|---|---|---|---|---|
| Upper-forward; lower-reverse | (°C) | Enzymes used | determination | |||
|
NF-α-308G > A (rs1800629) |
AGGCAATAGCTTTTGAGGGCCATG |
80 bp |
60 |
Sty I |
Intact |
:-308GG |
| TCTTCTGGGCCACTGACTGAT | ||||||
| 58 bp + 22 bp |
:-308GA + AA |
|||||
|
TNF-α-857C > T (rs1799724) |
CGAGTATGGGGACCCTCCCATTAA |
100 bp |
57 |
Ase1 |
Intact |
:-857CC |
| ACGTCCCCTGTATTCCATACC | ||||||
| 78 bp + 22 bp |
:-857CT + TT |
|||||
|
TNF-α-863C > A (rs1800630) |
GACCACAGCAATGGGTAGGAGA |
86 bp |
60 |
Bsl I |
Intact |
:-863CC |
| TCTACATGGCCCTGTCCTCGT | ||||||
| 64 bp + 22 bp |
:-863CA + AA |
|||||
|
TNF-α-1031T > C (rs1799964) |
AGAGCTGTGGGGAGAACAAA |
247 bp |
62 |
Bbs I |
Intact |
:-1031TT |
| CTCCTACCCATTGCTGTGGT | ||||||
| 127 bp + 120 bp | :-1031TC + CC | |||||