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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Nat Neurosci. 2013 Jun 23;16(8):1111–1117. doi: 10.1038/nn.3439

Figure 2. Reactivation of alcohol-associated memories increases levels of synaptic proteins.

Figure 2

A-C. Immunoblotting of mTORC1-regulated proteins in the amygdala (Amyg; A), mPFC (B) and OFC (C), 60 min after reactivation of alcohol-associated memory. A-C (left pane). The levels of Arc, GluR1, PSD-95 and NR were determined by western blot analysis and normalized to GAPDH. A-C (right pane). The memory reactivation-induced increase in Arc immunoreactivity was blocked by rapamycin (20 mg/kg, i.p) administered immediately after memory reactivation. Data are mean ± SEM and expressed as percentage of control. (A-C, left pane: t test; t’s(6)>2.50; *p<0.05, **p<0.01, A-C, right pane, Two-way ANOVA; Reactivation X Treatment interaction, [F(1, 12)>4.90, p<0.05], post-hoc comparisons **p<0.01; n=4 per group).