Skip to main content
. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Nat Neurosci. 2013 Jun 23;16(8):1111–1117. doi: 10.1038/nn.3439

Figure 3. Inhibition of mTORC1 after reactivation of alcohol-associated memories attenuates relapse measured as instrumental responding for alcohol.

Figure 3

A. Schematic representation of the experimental procedure. B, C & E. Data are mean ± SEM of active lever presses before abstinence (baseline), and during retention test and reacquisition stages. B. Effects of rapamycin (20 mg/kg ,i.p.) or vehicle given immediately after memory reactivation using presentation of context as well as odor-taste cue on lever presses during test and reacquisition. (Two-way ANOVA; Stage X Treatment interaction [F(2,22)=6.38, p<0.01]; post-hoc comparisons **p<0.01, n=12). C. Active and inactive lever presses during the test stage (Two-way ANOVA; Stage X Lever [F(1,22)=27.57, p<0.001]; post-hoc comparisons, active vs. inactive lever presses, **p<0.01 ***p<0.0001, n=12). D. Correlation plot of the number of lever presses during the reactivation session and the percentage of rapamycin-induced suppression in lever presses during the test (calculated as (presses in test / presses in baseline) X 100 in the rapamycin group). E. Effects of rapamycin (20 mg/kg, i.p) or vehicle, given 24 h before test without a reactivation session, on lever presses during test and reacquisition. (Two-way ANOVA; Stage X Treatment interaction [F(2,18)=0.53, p=0.59]; n=10).