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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Nat Neurosci. 2013 Jun 23;16(8):1111–1117. doi: 10.1038/nn.3439

Figure 5. Inhibition of mTORC1 after reactivation of alcohol-associated memories in the home cage induces a potent, long-term suppression of relapse.

Figure 5

A. Effects of rapamycin (20 mg/kg, i.p) or vehicle, given immediately after memory reactivation using an alcohol odor-taste cue in the home cage, on active lever presses during test and reacquisition (Left pane; Two-way ANOVA; Stage X Treatment interaction [F(2,26)=14.51, p<0.0001]; post-hoc comparisons *p<0.005, **p<0.001, n=8) and on active and inactive lever presses during the test stage (Right pane; Two-way ANOVA; Stage X Lever [F(1,13)=132.27, p<0.0001]; post-hoc comparisons, active vs. inactive lever presses, ***p<0.0001, n=8). Data are mean ± SEM of lever presses. B. Effects of rapamycin (20 mg/kg ,i.p.) or vehicle, given after memory reactivation on relapse to alcohol drinking in 2-bottle choice procedure. Data are mean ± SEM of alcohol intake (g/kg/24 h) during a 24 h 2-bottle choice session, in rapamycin- or vehicle-treated rats before abstinence (baseline), 24 h after reactivation, 14 d after reactivation, in the absence of reactivation, and 24 h after reactivation with a delayed (5 h) administration of rapamycin. (Two-way ANOVA, Condition X Treatment interaction [F(4, 106)=7.12, p<0.0001], post-hoc comparisons **p<0.001, n=8-12).