Fig. 2.

Fluorescence (a, c, e) and phase contrast (b, d, f) micrographs of human granulosa cells cultured with hCG (10 IU) at 0 h (a, b), 6 h (c, d), and 24 h (e, f). Cells were incubated with an antibody for AdipoR2 and imaged as described in materials and methods. The positive signal is seen as red. In Fig. 2g, at least 100 cells from 4 different cell isolations were imaged and counted for positive staining for AdipoR2 with defined thresholds as described in materials and methods. Bars represent averages with SEM. * represents significantly different from control (p < 0.05)