. 2013 Jun 19;30(7):865–875. doi: 10.1007/s10815-013-0028-8

Table 1.

Oocyte composition modifications and approaches used that may improve cryotolerance

Composition modification	Approach	Cell type/Species	Reference
Cytoplasmic lipid reduction	Micromanipulation/mechanical removal	Oocyte/Porcine	[7, 67]
Cytoplasmic lipid reduction	Media adjustment/pharmacologic manipulation	Embryo/Bovine	[7, 67]
Membrane fatty acid composition alteration	Liposomes	Oocyte, Sperm/Bovine	[4, 19, 101]
Membrane fatty acid composition alteration	Maturation medium supplementation	Oocyte, Sperm/Bovine	[4, 19, 101]
Membrane cholesterol content manipulation	Cyclodextran loading	Oocyte/Bovine	[43, 76]
Membrane aquaporin content	cRNA microinjection	Oocyte/Mouse	[97]