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. Author manuscript; available in PMC: 2013 Jul 28.
Published in final edited form as: Nat Immunol. 2006 Apr 9;7(5):498–506. doi: 10.1038/ni1327

Figure 2.

Figure 2

Cytokine production by pDCs. (a) IFN-α (left) and IL-6 (right) in 24-hour pDC culture supernatants of T-bet wild-type (T-bet WT) versus T-bet-deficient (T-bet KO) cells stimulated with various concentrations of CpG-B (ODN-1668). The pDCs were plated at a density of 1.0 × 106 cells/ml. (b) Comparison of secreted IFN-α protein, cellular IFN-α mRNA, secreted IL-6 and TNF (left to right) for Opn wild-type (Opn WT) and Opn-deficient (Opn KO) pDCs plated at a density of 1.0 × 106 cells/ml with CpG-B (1 μg/ml for mRNA samples; otherwise, concentrations along horizontal axes) and collected at 24 h. (c) Comparison of secreted IFN-α, IL-6 and TNF (left to right) for Opn wild-type (Opn WT) and Opn-deficient (Opn KO) pDCs after CpG-A stimulation. The pDCs (0.2 × 106 cells/ml) were plated with CpG-A (ODN-D19; concentration, horizontal axis) and were collected at 24 h for analysis. (d) IFN-α from 24-hour pDC culture supernatants (1.0 × 106 cells/ml) from Opn WT and Opn KO mice stimulated with imiquimod (R837). Protein and mRNA were measured by ELISA and real-time PCR in triplicate wells, except for c, for which the IFN-α ELISA was done in duplicate wells. Data are representative of two to three experiments.