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. Author manuscript; available in PMC: 2013 Jul 28.
Published in final edited form as: Opt Express. 2009 Feb 2;17(3):1282–1290. doi: 10.1364/oe.17.001282

Fig. 1.

Fig. 1

Schematic diagram of a multimodal NLO microscope using a fs laser source. The Mai Tai output at 790 nm (ω1) is split into two beams, with 80% of power being used to pump the Opal-BB. The idler output at 2036 nm from Opal-BB is doubled using a PPLN crystal. The rest of Mai Tai output is sent through a delay line and collinearly combined with the frequency-doubled idler beam at 1018 nm (ω2) for CARS imaging on a FV1000 laser-scanning inverted microscope. The 790 nm beam is also used for TPF and SHG imaging. The signal beam at 1290 nm (ω3) is used for SHG and THG imaging. Two internal spectral detectors are used for the microspectroscopy analysis. External detectors (not shown) are added for simultaneous forward and backward detection.