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| Stock | 10ml (1 gel) | 20 ml (2 gels) | |
| 4% acrylamide | 40% | 974 µl | 1.95 ml |
| 0.1% bis-acrylamide | 2% | 519 µl | 1.04 ml |
| 5 ml MgCl2 | 1 M | 50 µl | 100 µl |
| 1 mM ATP | 1 M | 10 µl | 20 µl (see CEB recipe) |
| 90 mM Tris-borate, pH 8.3 | 0.9 M | 1 ml | 2 ml |
| 2.5% Sucrose | 25% | 1 ml | 2 ml |
| Deionized H2O | 7.34 ml | 1.47 ml | |
| APS | 10% | 100 µl | 200 µl |
| TEMED | 10 µl | 20 µl |
