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. Author manuscript; available in PMC: 2013 Jul 28.
Published in final edited form as: Methods Mol Biol. 2012;832:349–362. doi: 10.1007/978-1-61779-474-2_24
Stock 5ml (1 gel) 10 ml (2 gels)
3% acrylamide 40% 300 µl 600 µl
0.3% bis-acrylamide 2% 1.5 ml 3 ml
5 mM MgCl2 1 M 25 µl 50 µl
1 mM ATP 1 M 5 µl 10 µl (see CEB recipe)
50 mM Tris-HCl, pH 6.8 1M 250 µl 500 µl
2.5% sucrose 25% 500 µl 1 ml
dH2O 2.4 ml 4.7 ml
APS 10% 50 µl 100 µl
TEMED 5 µl 10 µl